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Objective::To study the effect of Yupingfeng granule on the degranulation of skin mast cells in chronic urticaria (CU) rats and the intervention mechanism of interleukin-23(IL-23), interleukin-17(IL-17) inflammation axis. Method::Totally 60 SPF SD rats were selected and randomly divided into normal group (normal saline), model group (normal saline), and loratadine group (0.9 mg·kg-1·d-1), high-dose Yupingfeng granules group (4.05 g·kg-1·d-1), middle-dose group (2.7 g·kg-1·d-1), low-dose group (1.35 g·kg-1·d-1). The CU rat model was reproduced through intraperitoneal injection of ovalbumin with aluminum hydroxide suspension and DTP vaccine. Histopathological changes of rat skin were observed by hematoxylin-eosin (HE) staining. Degranulation of mast cells in rat skin was determined by toluidine blue staining. IL-23 and IL-17 protein expressions in skin tissue were determined by immunohistochemistry (IHC). IL-23 and IL-17 mRNA transcription levels in skin tissue were determined by real-time fluorescence quantitative polymerase chain reaction (Real-time PCR). Result::Yupingfeng granules can significantly alleviate the pathological manifestations of dermal edema, collagen beam distance, inflammatory cell infiltration of CU rats, and reduce the degranulation reaction of skin tissue mast cells in CU rats. The IL-23, IL-17 mRNA and protein expressions of the skin of model group were significantly increased compared with the normal group (P<0.05, P<0.01). Compared with the model group, Yupingfeng granules can significantly down-regulate IL-23 mRNA and protein expressions of CU rats (P<0.05, P<0.01). Yupingfeng granules had no significant regulatory effect on IL-17. Conclusion::Yupingfeng granule can significantly reduce the degranulation of mast cells in skin tissue of CU rats, and improve the pathological manifestations, such as dermal edema, serous exudation and inflammatory cell infiltration. The mechanism may be related to inhibiting the secretion of IL-23 pro-inflammatory cytokines and improving CU lesions.
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Objective:To discuss the clinical efficacy of comprehensive optimization of rehabilitation measures of traditional Chinese medicine(TCM) on elderly patients with COPD at stable period, in order to study its effect on immune function and inflammatory factors. Method:One hundred and forty-four patients were randomly divided into control group and observation group by random number table. Patients in control group got ipratropium bromide, 40-80 μg/time, 2-4 times/days, salmeterol roticasone powder, 1 suction/time, 2 times/days, as well as comprehensive western medical treatment measures of healthcare education, oxygen therapy and respiratory muscle exercise. In addition to the basic therapy of ipratropium bromide and salmeterol roticasone powder, patients in observation group was added with syndrome differentiation therapy of TCM, foot bath with TCM and respiratory function exercise with TCM. The course of treatment was 6 months, and 6-month follow-up were recorded. Comprehensive assessment of COPD was recorded. Before and after treatment, respiratory questionnaire (mMRC), self-assessment test for patients with chronic obstructive pulmonary disease (CAT), forced expiratory volume in the first second (FEV1), FEV1%, St George's respiratory questionnaire (SGRQ), 6-minute walking distance (6 MWD) were scored. And levels of CD3+, CD4+, CD8+, CD4+/CD8+, interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha) and hypersensitive C-reactive protein (hs-CRP) were detected. Result:Comprehensive assessment of COPD in the two groups were better than those before treatment (PZ=2.066, Pα were lower than those in control group (P+ was lower than that in control group (P1 and FEV1% were higher than those in control group (P+, CD4+, CD4+/CD8+ and 6-minute walking distance were more than those in control group (PConclusion:Comprehensive optimization of rehabilitation measures in TCM can inhibit inflammatory factors, improve immune function, alleviate clinical symptoms, improve lung function, improve exercise endurance and quality of life of patients, and promote the lung rehabilitation.
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Objective To investigate the effect ofYupingfeng granule combined with Desloratadine Citrate Disodium tablets on the clinical efficacy and immune function in patients with allergic rhinitis.Methods A total of 112 patients with allergic rhinitis were randomly divided into two groups according to their order of treatment. The patients in the control group (n=56) were treated with Desloratadine Citrate Disodium tablets, and the patients in the experimental group (n=56) were treated withYupingfeng granule and Desloratadine Citrate Disodium tablets. The efficacy of clinical, the rate of adverse reaction, the improvement of immune and the rate of recurrence were compared between the two groups. Results The total effective rate of the experimental group was 94.64%, it is higher than the control group 85.71%, and the difference was statistically significant (Z=-3.254,P<0.01). After the treatment, runny nose (1.2 ± 0.1vs. 2.0 ± 0.2,t=6.420), nasal itching (1.2 ± 0.3vs. 1.7 ± 0.3,t=7.003), sneezing (0.8 ± 0.1vs. 1.3 ± 0.1,t=6.716), stuffy nose (1.1 ± 0.1vs. 1.6 ± 0.3,t=7.256)scores in the experimental group were significantly lower than those in the control group (P<0.05). There was no significant difference in the total IgE (114.48 ± 9.85 IU/mlvs. 130.64 ± 10.51 IU/ml,t=6.994) and VCAM-1(512.56 ± 13.84 μg/Lvs. 624.98 ± 7.06 μg/L,t=7.803) between the two groups (P<0.05). The levels of IL-4(16.25 ± 1.53 ng/Lvs. 22.38 ± 3.16 ng/L,t=5.458), IL-10 (15.98 ± 2.43 ng/Lvs. 21.82 ± 1.75 ng/L, t=6.149), LTB4 (59.14 ± 0.96 ng/Lvs. 67.46 ± 3.04 ng/L,t=5.342) in the experimental group were significantly lower than those in the control group (P<0.05). Six-month after the treatment, the relapse rate of the experimental group was 5.36%, while the control group was 16.07%, and the difference between two groups were significant (P<0.05).Conclusions The combination ofYupingfeng granule and Desloratadine Citrate Disodium tablets in the treatment of allergic rhinitis can significantly improve the clinical symptoms, adjust the immune function and reduce the recurrence of the disease.
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Objective To investigate the effect ofYupingfeng granule combined with Desloratadine Citrate Disodium tablets on the clinical efficacy and immune function in patients with allergic rhinitis.Methods A total of 112 patients with allergic rhinitis were randomly divided into two groups according to their order of treatment. The patients in the control group (n=56) were treated with Desloratadine Citrate Disodium tablets, and the patients in the experimental group (n=56) were treated withYupingfeng granule and Desloratadine Citrate Disodium tablets. The efficacy of clinical, the rate of adverse reaction, the improvement of immune and the rate of recurrence were compared between the two groups. Results The total effective rate of the experimental group was 94.64%, it is higher than the control group 85.71%, and the difference was statistically significant (Z=-3.254,P<0.01). After the treatment, runny nose (1.2 ± 0.1vs. 2.0 ± 0.2,t=6.420), nasal itching (1.2 ± 0.3vs. 1.7 ± 0.3,t=7.003), sneezing (0.8 ± 0.1vs. 1.3 ± 0.1,t=6.716), stuffy nose (1.1 ± 0.1vs. 1.6 ± 0.3,t=7.256)scores in the experimental group were significantly lower than those in the control group (P<0.05). There was no significant difference in the total IgE (114.48 ± 9.85 IU/mlvs. 130.64 ± 10.51 IU/ml,t=6.994) and VCAM-1(512.56 ± 13.84 μg/Lvs. 624.98 ± 7.06 μg/L,t=7.803) between the two groups (P<0.05). The levels of IL-4(16.25 ± 1.53 ng/Lvs. 22.38 ± 3.16 ng/L,t=5.458), IL-10 (15.98 ± 2.43 ng/Lvs. 21.82 ± 1.75 ng/L, t=6.149), LTB4 (59.14 ± 0.96 ng/Lvs. 67.46 ± 3.04 ng/L,t=5.342) in the experimental group were significantly lower than those in the control group (P<0.05). Six-month after the treatment, the relapse rate of the experimental group was 5.36%, while the control group was 16.07%, and the difference between two groups were significant (P<0.05).Conclusions The combination ofYupingfeng granule and Desloratadine Citrate Disodium tablets in the treatment of allergic rhinitis can significantly improve the clinical symptoms, adjust the immune function and reduce the recurrence of the disease.
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To investigate the effects of Yupingfeng granule (YPF) on immune factors of the rats with allergic rhinitis (AR) induced by ovalbumin(OVA). OVA 0.3 mg, Al(OH)3 30 mg and saline 1 mL were mixed and intraperitoneally injected for the initial immunization, 4% OVA 200 μg (50 μL) was given to the nose on the 15th day for the second immunization to establish the allergic rhinitis model. Sixty male SD rats were randomly divided into allergic rhinitis(AR) model group, Yupingfeng granule three dose (2.7,1.35,0.68 g•kg⁻¹) groups, control drug Biyankang (0.4 g•kg⁻¹) and normal control group. After 14 days, efforts were made to collect blood from abdominal aorta, and take nasopharynx tissues and fasten them into 10% formaldehyde for a pathological examination. The levels of HIS, IgE, IL-4 and TNF-α in serum were examined by radioimmunoassay, and nasal mucosa tissues were examined by HE staining. According to the results, the levels of HIS, IgE, IL-4 and TNF-α in serum of Yupingfeng granule groups were significantly lower than that of AR model group (P<0.05, P<0.01). Nasal mucosa tissues showed slight morphological changes and inflammatory cell infiltration, with unobvious necrosis. Yupingfeng granule can improve the pathological changes of nasal mucosa tissues, and reduce the production and release of immune factors during allergic rhinitis (AR) process in vivo by OVA, which may be the important curative mechanism of allergic rhinitis.